how to do age-sex pyramid in excel

Say you have an age-sex breakdown and you want to summarise it in the usual way (as below). how do you do this in excel? this is definitely a post from the annals of ‘so ridiculous i had to write it down’. So, you have to give one of your sexes minus values, and then…

how to give a talk

I’m at IMMEM 2016 and there have been some great talks. However, some of them have been hampered by delivery, so i thought i would write a quick post on some things i try to do (a lot stolen from matt might and jen gardy). I used to have a job selling windows (made of…

Preparing a tree file for use with path-o-gen

Path-o-gen is a handy tool from Andy Rambaut’s group ‘for investigating the temporal signal and ‘clocklikeness’ of molecular phylogenies’. It is a common pre-cursor to the more involved BEAST analysis, to see whether there is any clock-like signal (i.e. SNPs accumulating with time) in the phylogeny. I wanted to do this today to look at…

IQTree vs RAxML for phylogenetic tree construction

This is a guest post from Anthony Underwood. ——————– I’ve been exploring the use of IQ-TREE for rapid ML tree calculation. I’ve had reservations for a while when using RAxML since even with multi-threading it can take hours to run and have often instead submitted them to the CIPRES supercomputing facilities at phylo.org where the…

NCTC3000 PacBio MLST

TL;DR I have run Torsten’s MLST script on the NCTC PacBio genomes, here are the results. I’m a massive fan of the NCTC3000 project, a collaboration between the National Collection of Type Cultures and the Wellcome Trust, to PacBio sequence 3000 bacterial type strains from the NCTC collection. One of the reasons I like the…

Weird SRA/ENA discrepancy

I’m trying to download some data from the Sequence Read Archive but it seems to be behaving quite unusually. Just writing a post to summarise this and see if anyone has seen it before. If I get an answer from the SRA helpdesk, I will post it in the comments/an edit. I’m interested in ERR024630, or…