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Monthly Archives: April 2014

Phylogenetic tree troubleshooting

Or, how I failed advanced file copying. TL:DR? Phylogenetic trees can be hard to interpret – use bootstraps to help! Low coverage in one strain or having an outlier can screw with your tree Including positions that have been ignored due to low quality in some of your strains can be useful (i.e. use RAxML and…

April 25, 2014 in Bioinformatics, How-to, Introduction, Microbiology.

IPython notebook of Salmonella e-burst groups

I thought it might be fun to knock up a quick IPython notebook with some information about the number of e-burst groups (eBGs) within some of the more common (in the UK) Salmonella serotypes. At the moment, it can only be statically rendered, via a github gist and the IPython notebook viewer. Might look further into hosting…

April 17, 2014 in Bioinformatics, Microbiology, Salmonella.

Sequence type of Salmonella enterica subsp. I reference genomes

As part of the on-going rollout of WGS at PHE, we needed to know the MLST Sequence Type (ST) of the completed Salmonella reference genomes. However, this info was not that easy to come across either in the literature or the Salmonella MLSTdb. I deteremined the ST of these genomes by converting the completed sequence…

April 4, 2014 in Microbiology, Salmonella.

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