IQTree vs RAxML for phylogenetic tree construction

This is a guest post from Anthony Underwood. ——————– I’ve been exploring the use of IQ-TREE for rapid ML tree calculation. I’ve had reservations for a while when using RAxML since even with multi-threading it can take hours to run and have often instead submitted them to the CIPRES supercomputing facilities at phylo.org where the…

NCTC3000 PacBio MLST

TL;DR I have run Torsten’s MLST script on the NCTC PacBio genomes, here are the results. I’m a massive fan of the NCTC3000 project, a collaboration between the National Collection of Type Cultures and the Wellcome Trust, to PacBio sequence 3000 bacterial type strains from the NCTC collection. One of the reasons I like the…

Weird SRA/ENA discrepancy

I’m trying to download some data from the Sequence Read Archive but it seems to be behaving quite unusually. Just writing a post to summarise this and see if anyone has seen it before. If I get an answer from the SRA helpdesk, I will post it in the comments/an edit. I’m interested inĀ ERR024630, or…