TL;DR Main I’m interested in identifying pseudogenes (also, and maybe more properly, known as hypothetically disrupted coding sequences [HDCs]) in some Salmonella genomes. There are two parts to this post, the “of general interest” part, which is an assessment of which tools work best for identifying pseudogenes, and a second more niche part. I tried…
I recently ran RGI on some gut microbiome samples, and I wanted to compare the prevalence of extended spectrum beta-lactamase (ESBL) genes between some of the groups in my study. This wasn’t listed directly in the RGI output, so I needed to add that info in. I asked around and found 3 solutions: Easy!
Subtitle: a series of sickening facts I’m interested in the role of the gut microbiome in infection, especially in settings with a high burden of infectious diseases. Therefore, I did a literature search on PubMed (full text of the search here). The search returned a total of 47 papers. What proportion do you think were…
I’m having a play around with some AI tools for literature searching and I came across the autoresearcher tool (link). Here is a comparison of the output of autoresearcher with ChatGPT4. TL;DR The question I asked both tools – “what is the role of the microbiome in mediating salmonella exposure outcomes?” autoresearcher output “The microbiome…
I was interested in getting a list of countries with a “high burden of infectious diseases”. From googling around, I found this nice image from Our World in Data (I love them! Source, with CSV etc). The underlying data from this comes from the Global Burden of Disease (GBD) initiative at the Institute of Health…
For some unfathomable reason some journals (e.g. Lancet journals) require you to use floating decimal places instead of normal ones. This is a real pain to do, because you can’t just do a find and replace because that would also replace the full stops. This is an opportunity to use a regex! ChatGPT tells us…
There have been some recent changes to the conda/mamba ecosystem, see obligatory xkcd. This is my current way of creating a fresh install of conda. Setting libmamba as the conda solver should allow you mamba-like speed, while at the same time letting you just install programs like `conda install`.
This is a “blog it so I don’t have to ask again on twitter post”. We have a narrative review that we want to post as a pre-print, but Medrxiv/Biorxiv don’t accept them. I asked for pre-print servers that do accept narrative reviews on twitter, and these are the sites that were recommended: SciELO OSF…
TL;DR SKA is a high quality bioinformatics tool which enables you to rapidly do genomic epidemiology of very closely related isolates. SKA shows good correlation with traditional SNP calling techniques (BWA + GATK) SKA shows a consistent trend of identifying around 10 more SNPs between a sub-set of pairs of isolates than traditional method, I’m…
This post is written in the spirit of “if you write it in an email to more than one person, turn it into a blog”. It’s for beginners who are just starting out in bioinformatics and who need information about which path to go down for which programming language. ————————— Firstly, you will need to…
Bits and Bugs 